Immunohistochemistry

We provide high-throughput IHC screening of normal and diseased tissues, using optimised IHC and ISH assays, HistologiX can detect the presence and amount of target within cell lines, patient tissue or xenografts. Examples of our services include the demonstration of protein expression for the cell signalling pathway, efficacy biomarkers, PK and PD study endpoints, POM and POP study biomarkers, pre- and post-therapy patient biopsy screening, pharmacology studies, bio-similar studies and companion diagnostic studies.

Histologix can provide multi-staining assays in order to demonstrate two or more targets in the same section.  Chromogenic methods are limited to two targets, while fluorescent method may identify up to four targets. Digital image analyses are especially valuable to identify co-staining in these assays.

Histologix use the Leica Bond Rx, Ventana Discovery and Benchmark, XT and Ultra automated IHC staining platforms and also LabVision systems.

Target Validation and Distribution

The use of tissue microarrays (TMAs) from specific disease types are a useful way to obtain relevant data from many samples, which can be combined with the necessary clinical history and patient follow-up data.

HistologiX also works closely with it’s sister company Tissue Solutions. Through their global network we can reliably and efficiently source valuable biological material adhering to the highest possible ethical standards. They offer a wide range of diseased and normal tissues in fresh, frozen, and FFPE formats.