Immunohistochemistry
Assay optimisation and high throughput screening for your studies
We provide high-throughput IHC screening of normal and diseased tissues, using optimised IHC and ISH assays, HistologiX can detect the presence and amount of target within cell lines, patient tissue or xenografts. Examples of our services include the demonstration of protein expression for the cell signalling pathway, efficacy biomarkers, PK and PD study endpoints, POM and POP study biomarkers, pre- and post-therapy patient biopsy screening, pharmacology studies, bio-similar studies and companion diagnostic studies.
Histologix can provide multi-staining assays in order to demonstrate two or more targets in the same section. Chromogenic methods are limited to two targets, while fluorescent method may identify up to four targets. Digital image analyses are especially valuable to identify co-staining in these assays.
Histologix use the Leica Bond Rx, Ventana Discovery and Benchmark, XT and Ultra automated IHC staining platforms and also LabVision systems.
Target Validation and Distribution
The use of tissue microarrays (TMAs) from specific disease types are a useful way to obtain relevant data from many samples, which can be combined with the necessary clinical history and patient follow-up data.
HistologiX also works closely with it’s sister company Tissue Solutions. Through their global network we can reliably and efficiently source valuable biological material adhering to the highest possible ethical standards. They offer a wide range of diseased and normal tissues in fresh, frozen, and FFPE formats.
IHC and IF Biomarker Development
Multiplexing
Target Validation and Distribution
Tissue Micro-Array Analysis
Ventana and Leica Autostainers
IHC Biomarkers
HistologiX offers assays for a range of commercially available IHC research biomarkers. Search our ever-growing list of biomarker assays below. Don’t see what you’re looking for? We can work with you to develop IHC biomarker assays for your specific biomarker. Get in touch to discuss your needs.
IHC Biomarkers | |
---|---|
Alpha smooth muscle actin antibody | Cytokeratin 10 |
Anti-PD1 | Cytokeratin 18 antibody |
Anti-PGP 9.5 | Epithelial sodium channel alpha antibody |
Bcl-xL (54H6) Rabbit mAb | ErbB 2 antibody |
BCL2 | Estrogen receptor alpha |
Beclin 1 | F4/80 |
Calponin | Filaggrin antibody |
Carbonic anyhydrase IX (CA9) | Fox-P3 |
CD11b | GFP |
CD137 |
GLP1R |
CD20 (EP459Y)(HRP) |
Granzyme B |
CD20 antibody – C-terminal | Grelin-1 |
CD24 |
Human Fibroblasts (TE-7) |
CD25 (IL-2) |
IL-2 |
CD3 |
IL-33 |
CD31 |
IL-8 |
CD32B | Ki67 |
CD38 antibody (SP149) C-terminal | LC3B |
CD4 | Lck (Phospho Y505) |
CD40 antibody | Mannose receptor antibody (CD206) |
CD56 | MCL-1 |
CD8 | MICA/B (F-6) |
CD8a | Muc 5B |
CEBP alpha antibody | NAK/TBK1 |
CFTR antibody | Newcastle’s Disease Virus |
Cleaved caspase 3 | NG2 |
Col I | OPTN/Optineurin |
Col III | PD-L1 |
Col IV | Phospho-Akt |
CONFIRM anti-CD3 (2GV6) | Podocin |
CONFIRM anti-CD4 (SP35) | Phospho-p38 MAPK (Thr180/Tyr182) |
CONFIRM anti-CD45RO (UCHL-1) | Progesterone Receptor antibody |
CONFIRM anti-CD8 (SP57) | PTEN antibody (Y184) |
Cytokeratin 1 | PTEN antibody (Y184) |