Immunohistochemistry

HistologiX provides high-throughput IHC screening of normal and diseased tissue using an extensive range of antibodies directed against cell signalling proteins.

Target Validation and Distribution

Using optimised IHC and ISH assays HistologiX can detect the presence of, and is able to quantify the levels of target within cell lines, patient tissue or xenografts. 

Using IHC or ISH it is possible to mine expression data from archive material of tumour populations. This will provide useful information when identifying therapy target or choosing the most likely antibody clones to develop as prognostic and predictive biomarkers.  The use of tissue microarrays (TMAs) from specific disease types are a useful way to obtain relevant data from many samples, which can be combined with the necessary clinical history and patient follow-up data.

Angiogenesis studies

In order to demonstrate the effects of antiangiogenic therapies, it is necessary to be able to stain and measure newly formed blood vessels.

By measuring the Micro Vessel Density (MVD) using our image analysis software we can identify changes to the size or number of stained blood vessels following therapy within given samples.

Cell Proliferation and Apoptosis studies

Abnormal cell proliferation is fundamental in tumourigenesis. Being able to identify and measure cell proliferation in pre and post therapeutic tissues is an important endpoint in determining drug therapy efficacy and in turn may be linked to apoptosis. 

A number of proliferative markers used for this purpose have been optimised by Histologix, along with quantitation using the Halo™ image analysis software will accurately and consistently measure proliferative index (% positive cells from a total of all cells).

BioSimilars

With a number of the original biotherapeutic molecules soon to be coming out of license there is a significant amount of interest in demonstrating similarities to generic versions of these drugs, eg. Rituximab, Avastin and Herceptin.

One of the many ways to demonstrate similarities between the generic and original versions is to generate xenografts from the relevant over-expressing tumour cell lines in SCID or Nude mice and to treat these with the relevant compounds using IHC/ISH to identify, quantify and compare data using Halo™ image analysis software.

Image Analysis

As technology evolves Histologix has invested internally and now offers the latest, most intuitive and highly functional image analysis software.

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